Group A rotavirus (RV-A) infections are a major cause of morbidity and mortality in human around the world. The aim of
this research was to conduct a molecular characterization of RV-A glycoprotein (G) and protease-sensitive (P) in urban
sewage (n=54; 27 raw sewage and 27 treated sewage) and sewage sludge (n=27) from Egypt by multiplex semi-nested
RT-PCR method. RV-A was detected in 29.9% of raw sewage, 7.4% of treated sewage, and 18.5% of sewage sludge
samples. In the positive samples, the RV-A G genotypes were as follow: G3 (n=6), G9 (n=3), G1 (n=2), G4 (n=1), G10 (n=1),
G1+G3 (n =1), and G1+G3+G10 (n=1) whereas P types detected included: P[4] (n=1), P[6] (n=3), P[8] (n=8), and
nontypeable P (n=3). The most detected G-P combination was G3P [8] (n=6). Other G-P combinations such as G1P [8]
(n=2), G9P [6] (n=2), G4P [6] (n=1), and G10P[4] (n=1) were also detected. The highest detection rates of RV-A in sewage
samples were found in winter (50%), followed by summer (25%), then in spring and autumn (12.5%). The results
showed not only that sewage analysis contains dramatic information concerning enteric viruses, but also that
environmental monitoring is an important approach in describing the local circulation of specific viruses among
population.
To know more go through the link
https://medwinpublishers.com/OAJMB/OAJMB16000141.pdf
To know more go through the link
https://medwinpublishers.com/OAJMB/OAJMB16000141.pdf
